Coding
RUNX2

Part:BBa_K415501

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-24)


RUNX2 L1L2 MammoBlock Entry Vector

RUNX2, or runt-related transcription factor, is a master osteoblast differentiation transcription factor; it is a downstream target of BMP-2 and switches on transcription of multiple effectors of osteoblast development. NCBI page with sequence, gene information etc. http://www.ncbi.nlm.nih.gov/gene/860

Isoforms: There are three isoforms of RUNX2 in humans, this part contains isoform Type-II, alpha, which is the longest and includes the 3' exon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 112
    Illegal PstI site found at 275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 112
    Illegal PstI site found at 275
    Illegal NotI site found at 291
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1533
    Illegal BamHI site found at 1366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 112
    Illegal PstI site found at 275
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 112
    Illegal PstI site found at 275
    Illegal NgoMIV site found at 83
    Illegal AgeI site found at 1400
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1403
    Illegal BsaI.rc site found at 1397
    Illegal SapI site found at 1295
    Illegal SapI.rc site found at 1086


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Categories
Parameters
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